Use the CODDLE link to start TILLING your gene, once you input the required information, Press the "CODDLE your gene button," a graphic with a score of missense and truncation of the target region will appear, press the "create primers for this window" button, a form called Primer3/Coddle, (Primer Picker) will appear. In most cases, use these CODDLE default parameters and press the "Pick Primers" button to proceed to the next step. A new form called Primers for your Gene will appear, showing the possible options, once you are satisfied with a set of primer’s parameters, click the "Display this pair of primers set" button, the next window (Your Gene), will show the Mutation Method, the polymorphisms that should be detected, and your primers, save this page (in html or word html format) and submit your order by e-mail as an attachment file to: meksemk@siu.edu. Are you ready? Press the "CODDLE Input" link to start! CODDLE Input http://www.proweb.org/coddle/ to obtain gene models and protein conservation models. http://tilling.fhcrc.org/parsesnp/ Analysis of identified mutations. http://www.proweb.org/glossary.html Descriptions of scoring missense changes. User Fees For current TILLING orders, please contact the soybean Tilling team for pricing and fees (meksemk@siu.edu.) For SOY-TP Update Contact us @ meksemk@siu.edu. Orders follow up Check TILLING Orders to get the status of orders Check TILLING Results to see sequencing results for an order Request Additional Results